Asymmetric Open-Closed Dimer Mechanism of Polyhydroxyalkanoate Synthase PhaC
نویسندگان
چکیده
منابع مشابه
Mutations derived from the thermophilic polyhydroxyalkanoate synthase PhaC enhance the thermostability and activity of PhaC from Cupriavidus necator H16.
The thermophile Cupriavidus sp. strain S-6 accumulated polyhydroxybutyrate (PHB) from glucose at 50°C. A 9.0-kbp EcoRI fragment cloned from the genomic DNA of Cupriavidus sp. S-6 enabled Escherichia coli XL1-Blue to synthesize PHB at 45°C. Nucleotide sequence analysis showed a pha locus in the clone. The thermophilic polyhydroxyalkanoate (PHA) synthase (PhaC(Csp)) shared 81% identity with mesop...
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We report data associated with the identification of three polyhydroxyalkanoate synthase genes (phaC) isolated from the marine bacteria metagenome of Aaptos aaptos marine sponge in the waters of Bidong Island, Terengganu, Malaysia. Our data describe the extraction of bacterial metagenome from sponge tissue, measurement of purity and concentration of extracted metagenome, polymerase chain reacti...
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Corresponding author † 14 Short title: Mutations enhancing the thermostability and activity of PHA synthase 15 Department of Marine Biotechnology, National Kaohsiung Marine University 16 Department of Seafood Science, National Kaohsiung Marine University 17 Mailing address: Department of Marine Biotechnology, National Kaohsiung Marine 18 University, No.142, Hai-Chuan Rd., Nan-Tzu Dist. Kaohsiun...
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Polyhydroxyalkanoates (PHAs) can be divided into three main types based on the sizes of the monomers incorporated into the polymer. Short-chain-length (SCL) PHAs consist of monomer units of C3 to C5, medium-chain-length (MCL) PHAs consist of monomer units of C6 to C14, and SCL-MCL PHAs consist of monomers ranging in size from C4 to C14. Although previous studies using recombinant Escherichia co...
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ژورنال
عنوان ژورنال: iScience
سال: 2020
ISSN: 2589-0042
DOI: 10.1016/j.isci.2020.101084